Coding
Part:BBa_K1842004
Designed by: Walter Thavarajah Group: iGEM15_UC_San_Diego (2015-09-17)
LuxDEC
The part codes for the fatty acid reductase complex of the bacterial lux system from Photobacterium Phosphoreum. The system recruits fatty acids and converts them into aldehyde to be used as substrate in the system's light producing reaction. The genes coding for each subunit of the complex are separated by a P2A sequence for stoichiometric expression. The part is comprised of three unique genes - LuxC, LuxD, and LuxE, coding for a reductase, transferase, and synthetase, respectively. Each gene has an attached antibody tag - LuxC has a FLAG tag, LuxD has a V5 tag, and LuxE has a HA tag. One copy of each gene is present. Genes used are codon optimized for expression in yeast.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1289
Illegal BglII site found at 2845
Illegal BamHI site found at 2046 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1723
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2334
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Categories
Parameters
None |